
Wild-type (WT) and PHD2 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHD2 antibody (GTX130482) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PHD2 antibody
GTX130482
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetEGLN1
Overview
- SupplierGeneTex
- Product NamePHD2 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.89 mg/ml
- ConjugateUnconjugated
- Gene ID54583
- Target nameEGLN1
- Target descriptionegl-9 family hypoxia inducible factor 1
- Target synonymsC1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6, egl nine homolog 1, HIF-prolyl hydroxylase 2, egl nine-like protein 1, hypoxia-inducible factor prolyl hydroxylase 2, prolyl hydroxylase domain-containing protein 2, zinc finger MYND domain-containing protein 6
- HostRabbit
- IsotypeIgG
- Protein IDQ9GZT9
- Protein NameEgl nine homolog 1
- Scientific DescriptionThe protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203







![PHD2 antibody detects PHD2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHD2 stained by PHD2 antibody (GTX132293) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX132293/GTX132293_42340_20171115_ICC_IF_R_w_23060523_568.webp)