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PHF1 Antibody (Preservative Free)

LS-C814976
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetPHF1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PHF1 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:1500 - 1:50000), IF (1:1500 - 1:50000), WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5252
  • Target name
    PHF1
  • Target description
    PHD finger protein 1
  • Target synonyms
    MTF2L2, PCL1, PHF2, TDRD19C, hPHF1, PHD finger protein 1, hPCl1, polycomb-like 1, polycomb-like protein 1, testicular tissue protein Li 140, tudor domain containing 19C
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHF1 using anti-PHF1 antibody (A03758-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF1 antigen affinity purified polyclonal antibody (Catalog # A03758-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF1 at approximately 62 kDa. The expected band size for PHF1 is at 62 kDa.
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