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ProductsBack/PHF21A / BHC80 Antibody (Preservative Free)

PHF21A / BHC80 Antibody (Preservative Free)

LS-C814977
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPHF21A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PHF21A / BHC80 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51317
  • Target name
    PHF21A
  • Target description
    PHD finger protein 21A
  • Target synonyms
    BHC80, BM-006, IDDBCS, NEDMS, PHD finger protein 21A, BHC80a, BRAF35-HDAC complex protein BHC80, BRAF35/HDAC2 complex (80 kDa)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHF21A using anti-PHF21A antibody (A08507-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates Lane 2: rat brain tissue lysates Lane 3: mouse brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF21A antigen affinity purified polyclonal antibody (Catalog # A08507-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF21A at approximately 75-95KD. The expected band size for PHF21A is at 75KD.
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