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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: NIH/3T3 cells, A431 cells, Primary antibody: CSB-PA794903(PHGDH Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: NIH/3T3 cells, A431 cells, Primary antibody: CSB-PA794903(PHGDH Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: NIH/3T3 cells, A431 cells, Primary antibody: CSB-PA794903(PHGDH Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute

PHGDH Antibody

CSB-PA794903
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPHGDH
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Overview

  • Supplier
    Cusabio
  • Product Name
    PHGDH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26227
  • Target name
    PHGDH
  • Target description
    phosphoglycerate dehydrogenase
  • Target synonyms
    2-oxoglutarate reductase; 3PGDH; 3-PGDH; 3-phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; HEL-S-113; malate dehydrogenase; NLS; NLS1; PDG; PGAD; PGD; PGDH; PHGDHD; SERA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43175
  • Protein Name
    D-3-phosphoglycerate dehydrogenase
  • Scientific Description
    This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Western blot All lanes: PHGDH antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa
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Figure 1. Western blot analysis of PHGDH using anti-PHGDH antibody (A03355-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHGDH antigen affinity purified polyclonal antibody (A03355-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHGDH at approximately 57 kDa. The expected band size for PHGDH is at 57 kDa.
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