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Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term (GTX101948). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (GTX101948). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term (GTX101948). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (GTX101948). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term (GTX101948). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (GTX101948). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

PHGDH antibody [N1N2], N-term

GTX101948
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPHGDH
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Overview

  • Supplier
    GeneTex
  • Product Name
    PHGDH antibody [N1N2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.87 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID26227
  • Target name
    PHGDH
  • Target description
    phosphoglycerate dehydrogenase
  • Target synonyms
    3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA, D-3-phosphoglycerate dehydrogenase, 2-oxoglutarate reductase, 3-phosphoglycerate dehydrogenase, epididymis secretory protein Li 113, malate dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43175
  • Protein Name
    D-3-phosphoglycerate dehydrogenase
  • Scientific Description
    3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hathazi D, Cox D, D'Amico A, et al. INPP5K and SIL1 associated pathologies with overlapping clinical phenotypes converge through dysregulation of PHGDH. Brain. 2021,144(8):2427-2442. doi: 10.1093/brain/awab133
    Read this paper

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