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Western blot analysis of 3T3 using p-ACCalpha (S80) antibody.
Western blot analysis of 3T3 using p-ACCalpha (S80) antibody.
Western blot analysis of 3T3 using p-ACCalpha (S80) antibody.

Phospho-ACACA (S80) Antibody

CSB-PA009195
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACACA
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-ACACA (S80) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID31
  • Target name
    ACACA
  • Target description
    acetyl-CoA carboxylase alpha
  • Target synonyms
    ACAC, ACACAD, ACACalpha, ACC, ACC1, ACCA, ACCalpha, Acac1, hACC1, acetyl-CoA carboxylase 1, ACC-alpha, acetyl-Coenzyme A carboxylase alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13085
  • Protein Name
    Acetyl-CoA carboxylase 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACACA using anti-ACACA antibody (A01802-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACACA antigen affinity purified polyclonal antibody (Catalog # A01802-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACACA at approximately 266 kDa. The expected band size for ACACA is at 266 kDa.
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