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Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody.The lane on the right is treated with the antigen-specific peptide.

Phospho-ARRB1 (Ser412) Antibody

CSB-PA045801
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetARRB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-ARRB1 (Ser412) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID408
  • Target name
    ARRB1
  • Target description
    arrestin beta 1
  • Target synonyms
    ARB1, ARR1, beta-arrestin-1, arrestin 2, non-visual arrestin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49407
  • Protein Name
    Beta-arrestin-1
  • Scientific Description
    Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation. Greg Buchanan. AACR Meeting Abstracts, Apr 2006; 2006: 430. D. Alex Groarke. Mol. Pharmacol., Feb 2001; 59: 375. Karen McConalogue. Mol. Biol. Cell, Aug 1998; 9: 2305. Maria Stella Lombardi. Stroke, Apr 2004; 35: 981 - 986.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARRB1 using anti-ARRB1 antibody (M02185). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whoel cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat PC-12 whoel cell lysates, Lane 6: mouse EL-4 whoel cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARRB1 antigen affinity purified monoclonal antibody (M02185) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARRB1 at approximately 50 kDa. The expected band size for ARRB1 is at 47 kDa.
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