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WB analysis of various samples using GTX02840 beta Arrestin 1 antibody [GT1243]. Dilution : 1:1000 Loading : 25microg
WB analysis of various samples using GTX02840 beta Arrestin 1 antibody [GT1243]. Dilution : 1:1000 Loading : 25microg
WB analysis of various samples using GTX02840 beta Arrestin 1 antibody [GT1243]. Dilution : 1:1000 Loading : 25microg

beta Arrestin 1 antibody [GT1243]

GTX02840
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetARRB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta Arrestin 1 antibody [GT1243] - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1243
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID408
  • Target name
    ARRB1
  • Target description
    arrestin beta 1
  • Target synonyms
    ARB1; ARR1; arrestin 2; beta-arrestin-1; non-visual arrestin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49407
  • Protein Name
    Beta-arrestin-1
  • Scientific Description
    Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARRB1 using anti-ARRB1 antibody (M02185). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whoel cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat PC-12 whoel cell lysates, Lane 6: mouse EL-4 whoel cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARRB1 antigen affinity purified monoclonal antibody (M02185) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARRB1 at approximately 50 kDa. The expected band size for ARRB1 is at 47 kDa.
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