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ProductsBack/Phospho-CDK1/2/3-T14 Rabbit mAb

Phospho-CDK1/2/3-T14 Rabbit mAb

AP1138
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDK1
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-CDK1/2/3-T14 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID983
  • Target name
    CDK1
  • Target description
    cyclin dependent kinase 1
  • Target synonyms
    CDC2, CDC28A, P34CDC2, cyclin-dependent kinase 1, cell cycle controller CDC2, cell division control protein 2 homolog, cell division cycle 2, G1 to S and G2 to M, cell division protein kinase 1, p34 protein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06493
  • Protein Name
    Cyclin-dependent kinase 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CDK1 using anti-CDK1 antibody (PB9533). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Spleen Tissue Lysate at 50ug, Lane 3: MCF-7 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: JURKAT Whole Cell Lysate at 40ug, Lane 6: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 antigen affinity purified polyclonal antibody (Catalog # PB9533) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)?with Tanon 5200 system. A specific band was detected for CDK1 at approximately 34 kDa. The expected band size for CDK1 is at 34 kDa.
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