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Phospho-GSK3alpha-S21+GSK3beta-S9 Rabbit mAb

AP1341
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetGSK3A
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-GSK3alpha-S21+GSK3beta-S9 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2931
  • Target name
    GSK3A
  • Target description
    glycogen synthase kinase 3 alpha
  • Target synonyms
    glycogen synthase kinase-3 alpha, GSK-3 alpha, serine/threonine-protein kinase GSK3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49840
  • Protein Name
    Glycogen synthase kinase-3 alpha
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GSK3 alpha using anti-GSK3 alpha antibody (A03152-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: mouse testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 alpha antigen affinity purified polyclonal antibody (Catalog # A03152-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 alpha at approximately 51KD. The expected band size for GSK3 alpha is at 51KD.
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