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Phospho-GYS1-S641 Rabbit mAb

AP1129
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetGYS1
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-GYS1-S641 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2997
  • Target name
    GYS1
  • Target description
    glycogen synthase 1
  • Target synonyms
    GSY, GYS, glycogen [starch] synthase, muscle, glycogen synthase 1 (muscle)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13807
  • Protein Name
    Glycogen [starch] synthase, muscle
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Glycogen synthase 1/GYS1 using anti-Glycogen synthase 1/GYS1 antibody (A03512-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycogen synthase 1/GYS1 antigen affinity purified polyclonal antibody (Catalog # A03512-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycogen synthase 1/GYS1 at approximately 84-90 kDa. The expected band size for Glycogen synthase 1/GYS1 is at 84 kDa.
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