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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8(Phospho-Ser39) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8(Phospho-Ser39) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8(Phospho-Ser39) Antibody(left) or the same antibody preincubated with blocking peptide(right).

Phospho-HDAC8 (Ser39) Antibody

CSB-PA795062
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHDAC8
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-HDAC8 (Ser39) Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55869
  • Target name
    HDAC8
  • Target description
    histone deacetylase 8
  • Target synonyms
    CDA07; CDLS5; HD8; HDACL1; histone deacetylase 8; histone deacetylase-like 1; KDAC8; MRXS6; RPD3; WTS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BY41
  • Protein Name
    Histone deacetylase 8
  • Scientific Description
    Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC8 gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. Lee H, et al. (2004) Mol Cell Biol. 24(2): 765-773.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HDAC8 using anti-HDAC8 antibody (A01843-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 antigen affinity purified polyclonal antibody (Catalog # A01843-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC8 at approximately 50 kDa. The expected band size for HDAC8 is at 42 kDa.
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