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Lane 1: Hela; Lane 2: K562 lysate probed with HDAC8 (4C3) Monoclonal Antibody (bsm-52088R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Hela; Lane 2: K562 lysate probed with HDAC8 (4C3) Monoclonal Antibody (bsm-52088R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Hela; Lane 2: K562 lysate probed with HDAC8 (4C3) Monoclonal Antibody (bsm-52088R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

HDAC8 Recombinant Antibody

BSM-52088R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetHDAC8
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HDAC8 (4C3) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), Flow-Cyt(1ug/Test), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID55869
  • Target name
    HDAC8
  • Target description
    histone deacetylase 8
  • Target synonyms
    CDA07; CDLS5; HD8; HDACL1; histone deacetylase 8; histone deacetylase-like 1; KDAC8; MRXS6; RPD3; WTS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BY41
  • Protein Name
    Histone deacetylase 8
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDAC8 using anti-HDAC8 antibody (A01843-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 antigen affinity purified polyclonal antibody (Catalog # A01843-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC8 at approximately 50 kDa. The expected band size for HDAC8 is at 42 kDa.
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