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Phospho-JAK2 (Tyr1007+Tyr1008) Antibody (YA181, Rabbit)

HY-P80467
MedChem Express
TargetJAK2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Phospho-JAK2 (Tyr1007+Tyr1008) Antibody (YA181, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3717
  • Target name
    JAK2
  • Target description
    Janus kinase 2
  • Target synonyms
    JTK10, tyrosine-protein kinase JAK2, JAK-2, Janus kinase 2 (a protein tyrosine kinase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Phospho-JAK2 (Tyr1007+Tyr1008) Antibody (YA181) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-JAK2 (Tyr1007+Tyr1008).
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of JAK2 using anti-JAK2 antibody (M00027). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK2 antigen affinity purified monoclonal antibody (Catalog # M00027) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK2 at approximately 131 kDa. The expected band size for JAK2 is at 131 kDa.
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