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ProductsBack/Phospho-PAK2-S20 Rabbit mAb

Phospho-PAK2-S20 Rabbit mAb

AP1443
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPAK2
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-PAK2-S20 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5062
  • Target name
    PAK2
  • Target description
    p21 (RAC1) activated kinase 2
  • Target synonyms
    KNO2, PAK65, PAKgamma, serine/threonine-protein kinase PAK 2, PAK-2, S6/H4 kinase, gamma-PAK, p21 (CDKN1A)-activated kinase 2, p21 protein (Cdc42/Rac)-activated kinase 2, p21-activated kinase 2, p58
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13177
  • Protein Name
    Serine/threonine-protein kinase PAK 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAK2 using anti-PAK2 antibody (A01419-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK2 antigen affinity purified polyclonal antibody (Catalog # A01419-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAK2 at approximately 58 kDa. The expected band size for PAK2 is at 58 kDa.
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