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Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody (left)or the same antibody preincubated with blocking peptide (right).
Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody (left)or the same antibody preincubated with blocking peptide (right).
Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody (left)or the same antibody preincubated with blocking peptide (right).

Phospho-PDPK1 (Tyr9) Antibody

CSB-PA781374
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDPK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-PDPK1 (Tyr9) Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5170
  • Target name
    PDPK1
  • Target description
    3-phosphoinositide dependent protein kinase 1
  • Target synonyms
    PDK1, PDPK2, PDPK2P, PRO0461, 3-phosphoinositide-dependent protein kinase 1, 3-phosphoinositide-dependent protein kinase 2 pseudogene, PkB kinase like gene 1, Putative 3-phosphoinositide-dependent protein kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15530
  • Protein Name
    3-phosphoinositide-dependent protein kinase 1
  • Scientific Description
    Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development. Alessi D.R., Curr. Biol. 7:261-269(1997). Alessi D.R.,Curr. Biol. 7:776-789(1997). Stephens L.R.,Science 279:710-714(1998)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: Mouse Liver lysates; Lane 2: Mouse Heart lysates probed with Phospho-PDPK1(Tyr373 + Tyr376) Polyclonal Antibody, Unconjugated (bs-3017R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of PDPK1 using anti-PDPK1 antibody (PB9735). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Lung Tissue Lysate, Lane 3: Mouse Liver Tissue Lysate, Lane 4: Mouse Lung Tissue Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: MCF-7 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody (Catalog # PB9735) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDPK1 at approximately 70KD. The expected band size for PDPK1 is at 63KD.
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