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ProductsBack/Phospho-PKA RIIalpha (PRKAR2A)-S99 Rabbit mAb

Phospho-PKA RIIalpha (PRKAR2A)-S99 Rabbit mAb

AP1034
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetPRKAR2A
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-PKA RIIalpha (PRKAR2A)-S99 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5576
  • Target name
    PRKAR2A
  • Target description
    protein kinase cAMP-dependent type II regulatory subunit alpha
  • Target synonyms
    PKR2, PRKAR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, cAMP-dependent protein kinase regulatory subunit RII alpha, protein kinase A, RII-alpha subunit, protein kinase, cAMP-dependent, regulatory subunit type II alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13861
  • Protein Name
    cAMP-dependent protein kinase type II-alpha regulatory subunit
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PKA R2/PKR2/PRKAR2A using anti-PKA R2/PKR2/PRKAR2A antibody (A03911-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mosue brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKA R2/PKR2/PRKAR2A antigen affinity purified polyclonal antibody (Catalog # A03911-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKA R2/PKR2/PRKAR2A at approximately 52 kDa. The expected band size for PKA R2/PKR2/PRKAR2A is at 52 kDa.
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