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Western Blot analysis of 3T3 cells using Phospho-c-Rel (S503) Polyclonal Antibody
Western Blot analysis of 3T3 cells using Phospho-c-Rel (S503) Polyclonal Antibody
Western Blot analysis of 3T3 cells using Phospho-c-Rel (S503) Polyclonal Antibody

Phospho-REL (S503) Antibody

CSB-PA040193
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetREL
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-REL (S503) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5966
  • Target name
    REL
  • Target description
    REL proto-oncogene, NF-kB subunit
  • Target synonyms
    C-Rel, HIVEN86A, IMD92, proto-oncogene c-Rel, oncogene REL, avian reticuloendotheliosis, v-rel avian reticuloendotheliosis viral oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04864
  • Protein Name
    Proto-oncogene c-Rel
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of c-Rel/REL using anti-c-Rel/REL antibody (A01880-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Rel/REL antigen affinity purified polyclonal antibody (Catalog # A01880-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Rel/REL at approximately 78 kDa. The expected band size for c-Rel/REL is at 69 kDa.
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