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ProductsBack/Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb

Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb

AP1518
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMAD1
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID4086
  • Target name
    SMAD1
  • Target description
    SMAD family member 1
  • Target synonyms
    BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1, mothers against decapentaplegic homolog 1, MAD, mothers against decapentaplegic homolog 1, Mad-related protein 1, SMAD, mothers against DPP homolog 1, TGF-beta signaling protein 1, mothers against DPP homolog 1, transforming growth factor-beta signaling protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15198
  • Protein Name
    Mothers against decapentaplegic homolog 9
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMAD1 using anti-SMAD1 antibody (A00728-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse kidney tissue lysate, Lane 5: mouse testis tissue lysate, Lane 6: human Hela cell lysate, Lane 7: human A375 cell lysate, Lane 8: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (Catalog # A00728-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD1 at approximately 52,60KD. The expected band size for SMAD1 is at 48, 52, 60KD.
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