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Untreated (–) and treated (+) Mouse tissue extract (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban (phospho Ser16) antibody (GTX132818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) Mouse tissue extract (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban (phospho Ser16) antibody (GTX132818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) Mouse tissue extract (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban (phospho Ser16) antibody (GTX132818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Phospholamban (phospho Ser16) antibody

GTX132818
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rabbit
TargetPLN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Phospholamban (phospho Ser16) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.97 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5350
  • Target name
    PLN
  • Target description
    phospholamban
  • Target synonyms
    CMD1P, CMH18, PLB, phospholamban, cardiac phospholamban
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26678
  • Protein Name
    Phospholamban
  • Scientific Description
    The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rabbit
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lee HL, Chang PC, Wo HT, et al. Beneficial Electrophysiological Effects of Rotigaptide Are Unable to Suppress Therapeutic Hypothermia-Provoked Ventricular Fibrillation in Failing Rabbit Hearts With Acute Ischemia-Reperfusion Injury. Front Physiol. 2021,12:726389. doi: 10.3389/fphys.2021.726389
    Read this paper

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Figure 1. Western blot analysis of Phospholamban/PLN using anti-Phospholamban/PLN antibody (A01395-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospholamban/PLN antigen affinity purified polyclonal antibody (Catalog # A01395-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospholamban/PLN at approximately 23 kDa. The expected band size for Phospholamban/PLN is at 6 kDa.
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