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Phospholipase A2, Calcium Independent (iPLA2) Polyclonal Antibody

CAU27951
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPLA2G6
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Overview

  • Supplier
    Biomatik
  • Product Name
    Phospholipase A2, Calcium Independent (iPLA2) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB; ICC; IHC-P; IHC-F; ELISA.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8398
  • Target name
    PLA2G6
  • Target description
    phospholipase A2 group VI
  • Target synonyms
    CaI-PLA2, GVI, INAD1, IPLA2-VIA, NBIA2, NBIA2A, NBIA2B, PARK14, PLA2, PNPLA9, iPLA2, iPLA2beta, 85/88 kDa calcium-independent phospholipase A2, 2-lysophosphatidylcholine acylhydrolase, 85 kDa calcium-independent phospholipase A2, GVI PLA2, iPLA2-beta, intracellular membrane-associated calcium-independent phospholipase A2 beta, neurodegeneration with brain iron accumulation 2, palmitoyl-CoA hydrolase, patatin-like phospholipase domain-containing protein 9, phospholipase A2, group VI (cytosolic, calcium-independent)
  • Host
    Rabbit
  • Protein IDO60733
  • Protein Name
    85/88 kDa calcium-independent phospholipase A2
  • Scientific Description
    The Phospholipase A2, Calcium Independent (iPLA2) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, ICC, IHC-P, IHC-F, ELISA.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLA2G6 using anti-PLA2G6 antibody (A01648-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G6 antigen affinity purified polyclonal antibody (Catalog # A01648-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G6 at approximately 90KD. The expected band size for PLA2G6 is at 90KD.
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