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WB analysis of rat brain lysate using GTX88536 PHR1 antibody, N-term. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat brain lysate using GTX88536 PHR1 antibody, N-term. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat brain lysate using GTX88536 PHR1 antibody, N-term. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer

PHR1 antibody, N-term

GTX88536
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetPLEKHB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PHR1 antibody, N-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1-3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID58473
  • Target name
    PLEKHB1
  • Target description
    pleckstrin homology domain containing B1
  • Target synonyms
    KPL1, PHR1, PHRET1, pleckstrin homology domain-containing family B member 1, PH domain containing, retinal 1, PH domain-containing family B member 1, PH domain-containing protein in retina 1, evectin-1, pleckstrin homology domain containing, family B (evectins) member 1, pleckstrin homology domain retinal protein 1
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9UF11
  • Protein Name
    Pleckstrin homology domain-containing family B member 1
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLEKHB1 using anti-PLEKHB1 antibody (A11037-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: monkey RF/6A whole cell lysates, Lane 5: rat eye tissue lysates, Lane 6: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHB1 antigen affinity purified polyclonal antibody (Catalog # A11037-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLEKHB1 at approximately 27 kDa. The expected band size for PLEKHB1 is at 27 kDa.
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