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PI3 Kinase p85 beta Antibody (YA688, Mouse)

HY-P80868
MedChem Express
TargetPIK3R2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PI3 Kinase p85 beta Antibody (YA688, Mouse)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5296
  • Target name
    PIK3R2
  • Target description
    phosphoinositide-3-kinase regulatory subunit 2
  • Target synonyms
    MPPH; MPPH1; p85; P85B; p85-BETA; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphoinositide-3-kinase regulatory subunit beta; phosphoinositide-3-kinase, regulatory subunit 2 (beta); PI3K regulatory subunit beta; PI3-kinase subunit p85-beta; ptdIns-3-kinase regulatory subunit p85-beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    PI3 Kinase p85 beta Antibody (YA688) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PI3 Kinase p85 beta.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIK3R2 using anti-PIK3R2 antibody (PB9777). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: 22RV1 Whole Cell Lysate, Lane 3: MCF-7 Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody (Catalog # PB9777) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85 kDa. The expected band size for PIK3R2 is at 85 kDa.
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