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Western Blot analysis of COS7 cells using PIASx Polyclonal Antibody
Western Blot analysis of COS7 cells using PIASx Polyclonal Antibody
Western Blot analysis of COS7 cells using PIASx Polyclonal Antibody

PIAS2 Antibody

CSB-PA003781
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetPIAS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PIAS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9063
  • Target name
    PIAS2
  • Target description
    protein inhibitor of activated STAT 2
  • Target synonyms
    androgen receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; E3 SUMO-protein ligase PIAS2; E3 SUMO-protein transferase PIAS2; MIZ1; msx-interacting zinc finger protein; PIASX; protein inhibitor of activated STAT X; SIZ2; zinc finger, MIZ-type containing 4; ZMIZ4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75928
  • Protein Name
    E3 SUMO-protein ligase PIAS2
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIAS2 using anti-PIAS2 antibody (A04130-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse testis tissue lysates, Lane 12: mouse Neuro-2a whole cell lysates, Lane 13: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS2 antigen affinity purified polyclonal antibody (Catalog # A04130-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS2 at approximately 68KD. The expected band size for PIAS2 is at 68KD.
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