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PIM1 Antibody (YA2502, Rabbit)

HY-P82757
MedChem Express
TargetPIM1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PIM1 Antibody (YA2502, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5292
  • Target name
    PIM1
  • Target description
    Pim-1 proto-oncogene, serine/threonine kinase
  • Target synonyms
    PIM, serine/threonine-protein kinase pim-1, Oncogene PIM1, pim-1 oncogene (proviral integration site 1), proto-oncogene serine/threonine-protein kinase pim-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PIM1 Antibody (YA2502) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2502), targeting PIM1, with a predicted molecular weight of 36 kDa (observed band size: 36 kDa). PIM1 Antibody (YA2502) can be used for WB, IHC-P experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIM1 using anti-PIM1 antibody (PB9315). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIM1 antigen affinity purified polyclonal antibody (Catalog # PB9315) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIM1 at approximately 44KD. The expected band size for PIM1 is at 44KD.
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