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WB analysis of extracts from HUVEC cells treated with PMA using GTX55311 PIM1 (phospho Tyr309) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
WB analysis of extracts from HUVEC cells treated with PMA using GTX55311 PIM1 (phospho Tyr309) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
WB analysis of extracts from HUVEC cells treated with PMA using GTX55311 PIM1 (phospho Tyr309) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide

PIM1 (phospho Tyr309) antibody

GTX55311
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetPIM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PIM1 (phospho Tyr309) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5292
  • Target name
    PIM1
  • Target description
    Pim-1 proto-oncogene, serine/threonine kinase
  • Target synonyms
    PIM, serine/threonine-protein kinase pim-1, Oncogene PIM1, pim-1 oncogene (proviral integration site 1), proto-oncogene serine/threonine-protein kinase pim-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11309
  • Protein Name
    Serine/threonine-protein kinase pim-1
  • Scientific Description
    The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIM1 using anti-PIM1 antibody (PB9315). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIM1 antigen affinity purified polyclonal antibody (Catalog # PB9315) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIM1 at approximately 44KD. The expected band size for PIM1 is at 44KD.
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