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Anti-PIN4 Antibody

CAB18173
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPIN4
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-PIN4 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID5303
  • Target name
    PIN4
  • Target description
    peptidylprolyl cis/trans isomerase, NIMA-interacting 4
  • Target synonyms
    EPVH, PAR14, PAR17, hEPVH, hPar14, hPar17, peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, PPIase PIN4, eukaryotic parvulin homolog, parvulin, parvulin-14, parvulin-17, peptidyl-prolyl cis-trans isomerase Pin4, peptidyl-prolyl cis/trans isomerase EPVH, protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin), rotamase PIN4
  • Host
    Rabbit
  • Protein IDQ9Y237
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
  • Scientific Description
    PIN4 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIN4 using anti-PIN4 antibody (M05181-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates. Lane 5: mouse liver tissue lysates. Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIN4 antigen affinity purified monoclonal antibody (Catalog # M05181-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIN4 at approximately 14 kDa. The expected band size for PIN4 is at 14 kDa.
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