ChIP analysis of human 5A8 J-Lat T lymphocytes culture treated with 10 microg/mL PHA (phytohemaglutin) for 0, 2, and 24 hours using GTX25816 PKA beta (catalytic subunit) (phospho Ser338) antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream and exon-1 of the EGR1 gene. IP reaction : 1.0ul antibody / 100ul sample
ChIP analysis of human 5A8 J-Lat T lymphocytes culture treated with 10 microg/mL PHA (phytohemaglutin) for 0, 2, and 24 hours using GTX25816 PKA beta (catalytic subunit) (phospho Ser338) antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream and exon-1 of the EGR1 gene. IP reaction : 1.0ul antibody / 100ul sample
PKA beta (catalytic subunit) (phospho Ser338) antibody
GTX25816
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
Product group Antibodies
ReactivityHuman, Mouse
TargetPRKACB
Overview
- SupplierGeneTex
- Product NamePKA beta (catalytic subunit) (phospho Ser338) antibody - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityPeptide competition data indicate that this antibody cross-reacts with the PKA nu subunit (64% homologous) and partially with the alpha subunit (82% homologous).
- Application Supplier NoteChIP assay: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID5567
- Target namePRKACB
- Target descriptionprotein kinase cAMP-activated catalytic subunit beta
- Target synonymsCAFD2; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PKACB; protein kinase A catalytic subunit beta; protein kinase, cAMP-dependent, beta catalytic subunit; protein kinase, cAMP-dependent, catalytic, beta
- HostRabbit
- IsotypeIgG
- Protein IDP22694
- Protein NamecAMP-dependent protein kinase catalytic subunit beta
- Scientific DescriptionThe protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203