More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PKA R2 (PRKAR2A) Mouse Monoclonal Antibody [Clone ID: OTI5F1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (RC220376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A (TA501195). Positive lysates LY401337 (100ug) and LC401337 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (RC220376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A (TA501195). Positive lysates LY401337 (100ug) and LC401337 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (RC220376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A (TA501195). Positive lysates LY401337 (100ug) and LC401337 (20ug) can be purchased separately from OriGene.

PKA R2 (PRKAR2A) Mouse Monoclonal Antibody [Clone ID: OTI5F1]

CF501195
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetPRKAR2A
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    PKA R2 (PRKAR2A) Mouse Monoclonal Antibody [Clone ID: OTI5F1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5F1
  • Gene ID5576
  • Target name
    PRKAR2A
  • Target description
    protein kinase cAMP-dependent type II regulatory subunit alpha
  • Target synonyms
    PKR2, PRKAR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, cAMP-dependent protein kinase regulatory subunit RII alpha, protein kinase A, RII-alpha subunit, protein kinase, cAMP-dependent, regulatory subunit type II alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP13861
  • Protein Name
    cAMP-dependent protein kinase type II-alpha regulatory subunit
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PRKAR2A mouse monoclonal antibody, clone OTI5F1 (formerly 5F1)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
RABGGTB AntibodyCAB15310
ReactivityHuman
TargetPRKAR2A
  • SizePrice
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate All lanes: PRKAR2A antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46, 44 kDa Observed band size: 46 kDa
Product group Antibodies
Cusabio
PRKAR2A AntibodyCSB-PA018697LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPRKAR2A
  • SizePrice
Anti-PRKAR2A Antibody
Product group Antibodies
Antibodies.com
Anti-PRKAR2A AntibodyA44417
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PRKAR2A AntibodyLS-C831768
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPRKAR2A
  • SizePrice
Product group Antibodies
MedChem Express
PKA R2/PKR2 Antibody (YA132, Rabbit)HY-P80429
TargetPRKAR2A
  • SizePrice
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Product group Antibodies
Atlas Antibodies
Anti-PRKAR2A AntibodyHPA038268
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPRKAR2A
  • SizePrice
Figure 1. Western blot analysis of PKA R2/PKR2/PRKAR2A using anti-PKA R2/PKR2/PRKAR2A antibody (A03911-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mosue brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKA R2/PKR2/PRKAR2A antigen affinity purified polyclonal antibody (Catalog # A03911-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKA R2/PKR2/PRKAR2A at approximately 52 kDa. The expected band size for PKA R2/PKR2/PRKAR2A is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-PKA R2/PKR2/PRKAR2A Antibody Picoband(r)A03911-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKAR2A
  • SizePrice
Product group Antibodies
Biomatik
Prkar2A Polyclonal AntibodyCAC08018
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetPRKAR2A
  • SizePrice
PKA R2 antibody detects PKA R2 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 10 % SDS-PAGE PKA R2 antibody (GTX103780) dilution: 1:1000
Product group Antibodies
GeneTex
PKA R2 antibodyGTX103780
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetPRKAR2A
  • SizePrice
Product group Antibodies
Bioss
PRKAR2A Recombinant Antbody Kit (KD-Validated)BSM-90468R
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman
TargetPRKAR2A
  • SizePrice