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PKC alpha antibody

GTX130453
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
DownloadDatasheet
Product group Antibodies
TargetPRKCA
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Overview

  • Supplier
    GeneTex
  • Product Name
    PKC alpha antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.25 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5578
  • Target name
    PRKCA
  • Target description
    protein kinase C alpha
  • Target synonyms
    AAG6; aging-associated gene 6; PKCA; PKC-A; PKCalpha; PKC-alpha; PKCI+/-; PRKACA; protein kinase C alpha type
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17252
  • Protein Name
    Protein kinase C alpha type
  • Scientific Description
    Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Neurokinin-1 receptor drives PKCalpha-AURKA/N-Myc signaling to facilitate the neuroendocrine progression of prostate cancer.
    Read more
  • Targeting A-kinase anchoring protein 12 phosphorylation in hepatic stellate cells regulates liver injury and fibrosis in mouse models. Ramani K et al., 2022 Oct 4, Elife
    Read more
  • PKCdelta mediates mitochondrial ROS generation and oxidation of HSP60 to relieve RKIP inhibition on MAPK pathway for HCC progression. Mandal JP et al., 2021 Feb 1, Free Radic Biol Med
    Read more
  • Toxoplasma gondii induces prolonged host epidermal growth factor receptor signalling to prevent parasite elimination by autophagy: Perspectives for in vivo control of the parasite. Lopez Corcino Y et al., 2019 Oct, Cell Microbiol
    Read more
  • Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation. Ramani K et al., 2018 Mar, Am J Pathol
    Read more
  • 18beta-glycyrrhetinic acid inhibits migration and invasion of human gastric cancer cells via the ROS/PKC-alpha/ERK pathway. Cai H et al., 2018 Jan, J Nat Med
    Read more

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Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-PKC alpha/beta II (Thr638/641) Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
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Figure 1. Western blot analysis of PKC Alpha/PRKCA using anti-PKC Alpha/PRKCA antibody (A00743-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Alpha/PRKCA antigen affinity purified polyclonal antibody (Catalog # A00743-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC Alpha/PRKCA at approximately 80 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.
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