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Western Blot analysis of MCF-7 cells using PKC Polyclonal Antibody
Western Blot analysis of MCF-7 cells using PKC Polyclonal Antibody
Western Blot analysis of MCF-7 cells using PKC Polyclonal Antibody

PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ Antibody

CSB-PA003800
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKCA
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Overview

  • Supplier
    Cusabio
  • Product Name
    PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5578
  • Target name
    PRKCA
  • Target description
    protein kinase C alpha
  • Target synonyms
    AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA, protein kinase C alpha type, PKC-A, aging-associated gene 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05129
  • Protein Name
    Protein kinase C gamma type
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-PKC alpha/beta II (Thr638/641) Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Product group Antibodies
References
Bioss
Phospho-PKC alpha (Thr638) Polyclonal AntibodyBS-3333R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPRKCA
  • SizePrice
Product group Antibodies
References
GeneTex
PKC alpha antibody [C1C3]GTX111096
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPRKCA
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-PKC alpha (T638) Antibody (YA163, Rabbit)HY-P80279
TargetPRKCA
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-PKC-alpha Antibody, Affinity PurifiedA302-445A
ApplicationsImmunoPrecipitation
TargetPRKCA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PRKCA Antibody144-00267
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetPRKCA
  • SizePrice
Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-PRKCA antibody. Corresponding PRKCA RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-PRKCA AntibodyHPA006564
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Figure 1. Western blot analysis of PKC Alpha/PRKCA using anti-PKC Alpha/PRKCA antibody (A00743-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Alpha/PRKCA antigen affinity purified polyclonal antibody (Catalog # A00743-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC Alpha/PRKCA at approximately 80 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.
Product group Antibodies
Boster Bio
Anti-PKC Alpha/PRKCA Antibody Picoband(r)A00743-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPRKCA
  • SizePrice
Product group Antibodies
Assay Genie
PKC alpha AntibodyCAB0267
TargetPRKCA
  • SizePrice
Product group Antibodies
Biomatik
Prkca Polyclonal AntibodyCAC09189
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetPRKCA
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PRKCA / PKC-Alpha AntibodyLS-C402927
ApplicationsWestern Blot, ELISA
TargetPRKCA
  • SizePrice