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Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (GTX107639) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (GTX107639) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (GTX107639) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PKC gamma antibody

GTX107639
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKCG
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Overview

  • Supplier
    GeneTex
  • Product Name
    PKC gamma antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.21 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5582
  • Target name
    PRKCG
  • Target description
    protein kinase C gamma
  • Target synonyms
    PKC-gamma, PKCC, PKCG, PKCI(3), PKCgamma, SCA14, protein kinase C gamma type
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05129
  • Protein Name
    Protein kinase C gamma type
  • Scientific Description
    Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhang X, Liu P, He X, et al. The PKCγ neurons in anterior cingulate cortex contribute to the development of neuropathic allodynia and pain-related emotion. Mol Pain. 2021,17:17448069211061973. doi: 10.1177/17448069211061973
    Read this paper
  • Liu P, Zhang X, He X, et al. Spinal GABAergic neurons are under feed-forward inhibitory control driven by Aδ and C fibers in Gad2 td-Tomato mice. Mol Pain. 2021,17:1744806921992620. doi: 10.1177/1744806921992620
    Read this paper

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Figure 1. Western blot analysis of PKC gamma using anti-PKC gamma antibody (A01890). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC gamma antigen affinity purified polyclonal antibody (Catalog # A01890) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC gamma at approximately 78 kDa. The expected band size for PKC gamma is at 78 kDa.
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