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PLA2G4A Rabbit pAb

A0394
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetPLA2G4A
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Overview

  • Supplier
    ABclonal
  • Product Name
    PLA2G4A Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5321
  • Target name
    PLA2G4A
  • Target description
    phospholipase A2 group IVA
  • Target synonyms
    GURDP, PLA2G4, cPLA2, cPLA2-alpha, cytosolic phospholipase A2, calcium-dependent phospholipid-binding protein, lysophospholipase, phosphatidylcholine 2-acylhydrolase, phospholipase A2, group IVA (cytosolic, calcium-dependent)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP47712
  • Protein Name
    Cytosolic phospholipase A2
  • Reactivity
    Human, Mouse
  • Reactivity Supplier Note
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLA2G4A using anti-PLA2G4A antibody (PB9778). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: NIH/3T3 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G4A antigen affinity purified polyclonal antibody (Catalog # PB9778) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G4A at approximately 85 kDa. The expected band size for PLA2G4A is at 85 kDa.
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