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Western blot analysis of extracts of Rat serum using PLA2G7 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Rat serum using PLA2G7 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Rat serum using PLA2G7 Polyclonal Antibody at 1:1000 dilution.

PLA2G7 Polyclonal Antibody

E-AB-65639
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PLA2G7 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAFAH using anti-PAFAH antibody (A02164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate, Lane 2: human SW620 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH antigen affinity purified polyclonal antibody (Catalog # A02164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAFAH at approximately 50KD. The expected band size for PAFAH is at 50KD.
Product group Antibodies
Boster Bio
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ApplicationsWestern Blot, ELISA
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TargetPLA2G7
  • SizePrice
Product group Antibodies
RayBiotech
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ReactivityHuman, Mouse, Rat
TargetPLA2G7
  • SizePrice
Product group Antibodies
Biomatik
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TargetPLA2G7
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAFAH Polyclonal Antibody, Unconjugated (bs-1451R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
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Bioss
PLA2G7 Polyclonal AntibodyBS-1451R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityCanine, Human, Mouse, Rat
TargetPLA2G7
  • SizePrice
Product group Antibodies
MedChem Express
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TargetPLA2G7
  • SizePrice
Product group Antibodies
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TargetPLA2G7
  • SizePrice
Product group Antibodies
GeneTex
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA08917A0Rb at dilution of 1:100
Product group Antibodies
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PLA2G7 AntibodyCSB-PA08917A0RB
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Everest Biotech
Goat anti-PLA2G7 (aa53-64) AntibodyEB11960
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Product group Antibodies
LifeSpan BioSciences
LP-PLA2 / PLA2G7 AntibodyLS-C748883
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPLA2G7
  • SizePrice