More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Placental Alkaline Phosphatase antibody
The data was published in the journal Sci Rep in 2019.
The data was published in the journal Sci Rep in 2019.
The data was published in the journal Sci Rep in 2019.

Placental Alkaline Phosphatase antibody

GTX72989
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetALPP
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Placental Alkaline Phosphatase antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Immunoblot 1:100-1:500. Immunoprecipitation, Immunohistochemistry 1:50. The optimal conditions should be determined by the individual laboratory. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 with AutoProbe III (GTX28620). It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID250
  • Target name
    ALPP
  • Target description
    alkaline phosphatase, placental
  • Target synonyms
    ALP, ALPI, IAP, PALP, PLAP, PLAP-1, alkaline phosphatase, placental type, Intestinal alkaline phosphatase, Intestinal-type alkaline phosphatase, alkaline phosphatase Regan isozyme, alkaline phosphomonoesterase, glycerophosphatase, placental alkaline phosphatase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05187
  • Protein Name
    Alkaline phosphatase, placental type
  • Scientific Description
    The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]
  • Reactivity
    Human, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Kohno T, Ishii K, Hirota Y, et al. Reelin-Nrp1 Interaction Regulates Neocortical Dendrite Development in a Context-Specific Manner. J Neurosci. 2020,40(43):8248-8261. doi: 10.1523/JNEUROSCI.1907-20.2020
    Read this paper
  • Satué M, Schüler C, Ginner N, et al. Intra-articularly injected mesenchymal stem cells promote cartilage regeneration, but do not permanently engraft in distant organs. Sci Rep. 2019,9(1):10153. doi: 10.1038/s41598-019-46554-5
    Read this paper
  • Zwolanek D, Satué M, Proell V, et al. Tracking mesenchymal stem cell contributions to regeneration in an immunocompetent cartilage regeneration model. JCI Insight. 2017,2(20):pii: 87322. doi: 10.1172/jci.insight.87322.
    Read this paper
  • Kohno T, Honda T, Kubo K, et al. Importance of Reelin C-terminal region in the development and maintenance of the postnatal cerebral cortex and its regulation by specific proteolysis. J Neurosci. 2015,35(11):4776-87. doi: 10.1523/JNEUROSCI.4119-14.2015
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of ALPP using anti-ALPP antibody (A01718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALPP antigen affinity purified polyclonal antibody (Catalog # A01718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALPP at approximately 70 kDa. The expected band size for ALPP is at 58 kDa.
Product group Antibodies
Boster Bio
Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody Picoband(r)A01718-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetALPP
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-PLAP [10G6D9E8]Ab03404-1.1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetALPP
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Alkaline phosphatase (ALPP) Antibody130-10128
  • SizePrice
Product group Antibodies
Biosynth
ALPP antibody10R-2198
  • SizePrice
Product group Antibodies
Biomatik
Alpp Polyclonal AntibodyCAC10640
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetALPP
  • SizePrice
Hela cell lysates probed with Placental alkaline phosphatase Polyclonal Antibody, Unconjugated (bs-20015R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss
Placental alkaline phosphatase (PLAP) Polyclonal AntibodyBS-20015R
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetALPP
  • SizePrice
Western blot All lanes: Alkaline phosphatase, placental type antibody at 8ug/ml Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Product group Antibodies
Cusabio
ALPP AntibodyCSB-PA001632ESR1HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetALPP
  • SizePrice
Product group Antibodies
GeneTex
Placental Alkaline Phosphatase antibody [ZM161]GTX01801
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetALPP
  • SizePrice
IHC-P analysis of human seminoma tissue using GTX01925 Placental Alkaline Phosphatase antibody [8A9]. Note the strong membrane and cytoplasmic staining of malignant cells.
Product group Antibodies
GeneTex
Placental Alkaline Phosphatase antibody [8A9]GTX01925
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetALPP
  • SizePrice
Product group Antibodies
GeneTex
Placental Alkaline Phosphatase antibody [H17E2]GTX11299
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman
TargetALPP
  • SizePrice