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WB analysis of HepG2 cells using GTX45179 PLCB1 antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX45179 PLCB1 antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX45179 PLCB1 antibody at 0.2-1microg/ml.

PLCB1 antibody, Internal

GTX45179
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPLCB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PLCB1 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23236
  • Target name
    PLCB1
  • Target description
    phospholipase C beta 1
  • Target synonyms
    1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1; DEE12; EIEE12; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; phosphoinositidase C; phosphoinositide phospholipase C; phospholipase C, beta 1 (phosphoinositide-specific); phospholipase C-I; PI-PLC; PLC154; PLC-154; PLCB1A; PLCB1B; PLC-beta-1; PLC-I; triphosphoinositide phosphodiesterase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQ66
  • Protein Name
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
  • Scientific Description
    The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLCB1 using anti-PLCB1 antibody (A02817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCB1 antigen affinity purified polyclonal antibody (Catalog # A02817-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCB1 at approximately 150 kDa. The expected band size for PLCB1 is at 138 kDa.
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