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ProductsBack/PLCB1 / Phospholipase C Beta 1 Antibody

PLCB1 / Phospholipase C Beta 1 Antibody

LS-C331795
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPLCB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PLCB1 / Phospholipase C Beta 1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 133kDa/138kDa, while the observed MW by Western blot was 145kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 133kDa/138kDa, while the observed MW by Western blot was 145kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23236
  • Target name
    PLCB1
  • Target description
    phospholipase C beta 1
  • Target synonyms
    DEE12, EIEE12, PI-PLC, PLC-154, PLC-I, PLC-beta-1, PLC154, PLCB1A, PLCB1B, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate, inositoltrisphosphohydrolase, monophosphatidylinositol phosphodiesterase, phosphoinositidase C, phosphoinositide phospholipase C, phospholipase C, beta 1 (phosphoinositide-specific), phospholipase C-I, triphosphoinositide phosphodiesterase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLCB1 using anti-PLCB1 antibody (A02817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCB1 antigen affinity purified polyclonal antibody (Catalog # A02817-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCB1 at approximately 150 kDa. The expected band size for PLCB1 is at 138 kDa.
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