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Western Blot analysis of HepG2 cells using PLC gamma1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using PLC gamma1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using PLC gamma1 Polyclonal Antibody

PLCG1 Antibody

CSB-PA003818
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetPLCG1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PLCG1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5335
  • Target name
    PLCG1
  • Target description
    phospholipase C gamma 1
  • Target synonyms
    IDAA, NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate, PLC-148, PLC-gamma-1, inositoltrisphosphohydrolase, monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphoinositidase C, phosphoinositide phospholipase C, phosphoinositide phospholipase C-gamma-1, phospholipase C, gamma 1 (formerly subtype 148), phospholipase C-148, phospholipase C-II, triphosphoinositide phosphodiesterase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19174
  • Protein Name
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLCG1 using anti-PLCG1 antibody (M00677). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCG1 antigen affinity purified monoclonal antibody (Catalog # M00677) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCG1 at approximately 149 kDa. The expected band size for PLCG1 is at 149 kDa.
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