Anti-PLC-gamma phosphorylated [AbAb1-PLCy]

Absolute Antibody

Anti-PLC-gamma phosphorylated [AbAb1-PLCy]

9

The binding reactivity of this antibody to recombinant phosphorylated pPLC-gamma peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of phosphorylated PLC-gamma from NIH3T3 cell lysates. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pPLC-gamma. The fluorescent scFv version of this antibody, when expressed intracellularly was reported to inhibit signal transduction by PLC-gamma in transfected into NIH3T3 cells as observed using fluorescent microscopy (US20090233358).

ImmunoPrecipitation, Western Blot, ELISA

Research Use Only

Monoclonal

AbAb1-PLCy

PLCG1

phospholipase C gamma 1

IDAA, NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate, PLC-148, PLC-gamma-1, inositoltrisphosphohydrolase, monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphoinositidase C, phosphoinositide phospholipase C, phosphoinositide phospholipase C-gamma-1, phospholipase C, gamma 1 (formerly subtype 148), phospholipase C-148, phospholipase C-II, triphosphoinositide phosphodiesterase

Human

IgG1

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

-20°C,2°C to 8°C

12352203