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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA174144(PLEC Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA174144(PLEC Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA174144(PLEC Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

PLEC Antibody

CSB-PA174144
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPLEC
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Overview

  • Supplier
    Cusabio
  • Product Name
    PLEC Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5339
  • Target name
    PLEC
  • Target description
    plectin
  • Target synonyms
    EBS1; EBSMD; EBSND; EBSO; EBSOG; EBSPA; HD1; hemidesmosomal protein 1; LGMD2Q; LGMDR17; PCN; PLEC1; PLEC1b; plectin; plectin 1, intermediate filament binding protein 500kDa; PLTN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15149
  • Protein Name
    Plectin
  • Scientific Description
    Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Plectin using anti-Plectin antibody (PB9430). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plectin antigen affinity purified polyclonal antibody (Catalog # PB9430) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plectin at approximately 532 kDa. The expected band size for Plectin is at 532 kDa.
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