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Plectin Antibody (YA2915, Rabbit)

HY-P83170
MedChem Express
TargetPLEC
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Plectin Antibody (YA2915, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5339
  • Target name
    PLEC
  • Target description
    plectin
  • Target synonyms
    EBS1, EBS5A, EBS5B, EBS5C, EBS5D, EBSMD, EBSND, EBSO, EBSOG, EBSPA, HD1, LGMD2Q, LGMDR17, PCN, PLEC1, PLEC1b, PLTN, plectin, hemidesmosomal protein 1, plectin 1, intermediate filament binding protein 500kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Plectin Antibody (YA2915) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2915), targeting Plectin, with a predicted molecular weight of 532 kDa (observed band size: 532 kDa). Plectin Antibody (YA2915) can be used for WB, IHC-P, IP experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Plectin using anti-Plectin antibody (PB9430). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plectin antigen affinity purified polyclonal antibody (Catalog # PB9430) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plectin at approximately 532 kDa. The expected band size for Plectin is at 532 kDa.
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