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WB analysis of various sample lysates using GTX33414 Pleckstrin antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33414 Pleckstrin antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33414 Pleckstrin antibody. Dilution : 1:1000 Loading : 25microg per lane

Pleckstrin antibody

GTX33414
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPLEK
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Overview

  • Supplier
    GeneTex
  • Product Name
    Pleckstrin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5341
  • Target name
    PLEK
  • Target description
    pleckstrin
  • Target synonyms
    P47, PLEK1, pleckstrin, platelet 47 kDa protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08567
  • Protein Name
    Pleckstrin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Shih TL, Lin KH, Chen RJ, et al. A novel naphthalimide derivative reduces platelet activation and thrombus formation via suppressing GPVI. J Cell Mol Med. 2021,25(19):9434-9446. doi: 10.1111/jcmm.16886
    Read this paper
  • Lee TY, Jayakumar T, Thanasekaran P, et al. Carbon Dot Nanoparticles Exert Inhibitory Effects on Human Platelets and Reduce Mortality in Mice with Acute Pulmonary Thromboembolism. Nanomaterials (Basel). 2020,10(7). doi: 10.3390/nano10071254
    Read this paper
  • Hsia CW, Velusamy M, Tsao JT, et al. New Therapeutic Agent against Arterial Thrombosis: An Iridium(III)-Derived Organometallic Compound. Int J Mol Sci. 2017,18(12). doi: 10.3390/ijms18122616
    Read this paper
  • Hsia CH, Velusamy M, Sheu JR, et al. A novel ruthenium (II)-derived organometallic compound, TQ-6, potently inhibits platelet aggregation: Ex vivo and in vivo studies. Sci Rep. 2017,7(1):9556. doi: 10.1038/s41598-017-09695-z
    Read this paper
  • Lien LM, Lin KH, Huang LT, et al. Licochalcone A Prevents Platelet Activation and Thrombus Formation through the Inhibition of PLCγ2-PKC, Akt, and MAPK Pathways. Int J Mol Sci. 2017,18(7). doi: 10.3390/ijms18071500
    Read this paper

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Figure 1. Western blot analysis of Pleckstrin/PLEK using anti-Pleckstrin/PLEK antibody (A06656-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human U937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pleckstrin/PLEK antigen affinity purified polyclonal antibody (Catalog # A06656-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pleckstrin/PLEK at approximately 40 kDa. The expected band size for Pleckstrin/PLEK is at 40 kDa.
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