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Pleckstrin Antibody (YA3163)

HY-P83418
MedChem Express
TargetPLEK
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Pleckstrin Antibody (YA3163)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5341
  • Target name
    PLEK
  • Target description
    pleckstrin
  • Target synonyms
    P47; platelet 47 kDa protein; pleckstrin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Pleckstrin Antibody (YA3163) is a non-conjugated IgG antibody, targeting Pleckstrin, with a predicted molecular weight of 40 kDa (observed band size: 40 kDa). Pleckstrin Antibody (YA3163) can be used for WB experiment in human background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Pleckstrin/PLEK using anti-Pleckstrin/PLEK antibody (A06656-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human U937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pleckstrin/PLEK antigen affinity purified polyclonal antibody (Catalog # A06656-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pleckstrin/PLEK at approximately 40 kDa. The expected band size for Pleckstrin/PLEK is at 40 kDa.
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