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ProductsBack/PLGF antibody [HL2682]

PLGF antibody [HL2682]

GTX639346
GeneTex
ApplicationsELISA
Product group Antibodies
TargetPGF
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Overview

  • Supplier
    GeneTex
  • Product Name
    PLGF antibody [HL2682]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2682
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5228
  • Target name
    PGF
  • Target description
    placental growth factor
  • Target synonyms
    D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760, placenta growth factor, placental growth factor, vascular endothelial growth factor-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49763
  • Protein Name
    Placenta growth factor
  • Scientific Description
    This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PGF using anti-PGF antibody (A01164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGF antigen affinity purified polyclonal antibody (Catalog # A01164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGF at approximately 25KD. The expected band size for PGF is at 25KD.
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