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PLIN Antibody (OAEB01208)

OAEB01208
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetPLIN1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    PLIN Antibody (OAEB01208)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
  • Gene ID5346
  • Target name
    PLIN1
  • Target description
    perilipin 1
  • Target synonyms
    FPLD4; lipid droplet-associated protein; PERI; perilipin-1; PLIN
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Perilipin A using anti-Perilipin A antibody (A03918-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Perilipin A antigen affinity purified polyclonal antibody (Catalog # A03918-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Perilipin A at approximately 62KD. The expected band size for Perilipin A is at 56KD.
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