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ProductsBack/PLVAP antibody [174/2]

PLVAP antibody [174/2]

GTX54474
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetPLVAP
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Overview

  • Supplier
    GeneTex
  • Product Name
    PLVAP antibody [174/2]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    174/2
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID83483
  • Target name
    PLVAP
  • Target description
    plasmalemma vesicle associated protein
  • Target synonyms
    DIAR10, FELS, PV-1, PV1, gp68, plasmalemma vesicle-associated protein, fenestrated endothelial-linked structure protein, fenestrated-endothelial linked structure protein; PV-1 protein, plasmalemma vesicle protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9BX97
  • Protein Name
    Plasmalemma vesicle-associated protein
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLVAP using anti-PLVAP antibody (A07024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLVAP antigen affinity purified polyclonal antibody (Catalog # A07024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLVAP at approximately 53 kDa. The expected band size for PLVAP is at 51 kDa.
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