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ProductsBack/Plxdc2 antibody [N1C1]

Plxdc2 antibody [N1C1]

GTX118575
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetPLXDC2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Plxdc2 antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.95 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID84898
  • Target name
    PLXDC2
  • Target description
    plexin domain containing 2
  • Target synonyms
    PLXDC2-OT, TEM7R, plexin domain-containing protein 2, 1200007L24Rik, tumor endothelial marker 7-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UX71
  • Protein Name
    Plexin domain-containing protein 2
  • Scientific Description
    May play a role in tumor angiogenesis.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Shimizu H, Nakagawa M, Todaka N, et al. Improving the quality of a recombinant rabbit monoclonal antibody against PLXDC2 by optimizing transient expression conditions and purification method. Protein Expr Purif. 2018,146:27-33. doi: 10.1016/j.pep.2018.01.011
    Read this paper

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Figure 1. Western blot analysis of PLXDC2 using anti-PLXDC2 antibody (A08798-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLXDC2 antigen affinity purified polyclonal antibody (Catalog # A08798-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLXDC2 at approximately 60 kDa. The expected band size for PLXDC2 is at 60 kDa.
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