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ProductsBack/PLXNC1 Antibody - C-terminal region

PLXNC1 Antibody - C-terminal region

OAAB09877
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetPLXNC1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    PLXNC1 Antibody - C-terminal region
  • Delivery Days Customer
    23
  • Application Supplier Note
    WB: 1:1000 IHC-P: 1:10~50
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Gene ID10154
  • Target name
    PLXNC1
  • Target description
    plexin C1
  • Target synonyms
    CD232, PLXN-C1, VESPR, plexin-C1, plexin (semaphorin receptor), receptor for viral semaphorin protein, receptor for virally-encoded semaphorin, virus-encoded semaphorin protein receptor
  • Host
    Rabbit
  • Protein IDO60486
  • Protein Name
    Plexin-C1
  • Scientific Description
    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLXNC1 using anti-PLXNC1 antibody (A10941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLXNC1 antigen affinity purified polyclonal antibody (Catalog # A10941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLXNC1 at approximately 220 kDa. The expected band size for PLXNC1 is at 176 kDa.
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