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Western Blot analysis of HeLa cells using PMS2 Polyclonal Antibody
Western Blot analysis of HeLa cells using PMS2 Polyclonal Antibody
Western Blot analysis of HeLa cells using PMS2 Polyclonal Antibody

PMS2 Antibody

CSB-PA003827
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPMS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PMS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5395
  • Target name
    PMS2
  • Target description
    PMS1 homolog 2, mismatch repair system component
  • Target synonyms
    HNPCC4, LYNCH4, MLH4, MMRCS4, PMS-2, PMS2CL, PMSL2, mismatch repair endonuclease PMS2, DNA mismatch repair protein PMS2, PMS1 homolog 2, mismatch repair protein, PMS1 protein homolog 2, PMS2 postmeiotic segregation increased 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54278
  • Protein Name
    Mismatch repair endonuclease PMS2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PMS2 using anti-PMS2 antibody (A01028). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMS2 antigen affinity purified polyclonal antibody (Catalog # A01028) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMS2 at approximately 115KD. The expected band size for PMS2 is at 96KD.
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