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PMS2CL/PMS2 Antibody

PACO04625
Assay Genie
ApplicationsELISA, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetPMS2
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PMS2CL/PMS2 Antibody (PACO04625)
  • Delivery Days Customer
    9
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IHC, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5395
  • Target name
    PMS2
  • Target description
    PMS1 homolog 2, mismatch repair system component
  • Target synonyms
    DNA mismatch repair protein PMS2; HNPCC4; mismatch repair endonuclease PMS2; MLH4; MMRCS4; PMS1 homolog 2, mismatch repair protein; PMS1 protein homolog 2; PMS2 postmeiotic segregation increased 2; PMS2CL; PMSL2; postmeiotic segregation increased 2 nirs variant 6
  • Host
    Rabbit
  • Protein IDP54278
  • Protein Name
    Mismatch repair endonuclease PMS2
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PMS2 using anti-PMS2 antibody (A01028). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMS2 antigen affinity purified polyclonal antibody (Catalog # A01028) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMS2 at approximately 115KD. The expected band size for PMS2 is at 96KD.
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